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Genetically Engineered Proteins | |
| Biosensing and Diagnostic Systems | ||
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Products > Photoproteins > Aquamax Product Application/Background:The photoprotein aequorin, originally isolated from the jellyfish Aequoria victoria is calcium binding bioluminescent protein. The light emission from aequorin is dependent on calcium concentration, and it is this property that has allowed its use in the determination of intracellular calcium in various cell compartments. Aequorin is also being employed as a highly sensitive label in binding assays, as it can be detected down to attomole levels. The use of aequorin allows highly sensitive detection due to the virtual absence of background bioluminescence signal. AquamaxTM Aequorin is a recombinant form of aequorin. The apoaequorin is produced in E. coli and then charged with coelenterazine to produce the active photoprotein. The bioluminescence kinetic profile of Aquamax is nearly identical to that of the naturally occurring aequorin.
Aquamax can be detected down to 1 x 10-14 M (subattomole range) and its dynamic range spans over 7 orders of magnitude (see Figure above). This makes it an ideal reporter/label for diagnostic applications.
Aquamax by itself can be employed in calcium sensing, as a label in immunoassays/binding assays, in single molecule detection, and for the analysis of biomolecules in body fluids without any pretreatment. Moreover, SenseOmics genetically modifies Aquamax by chemical or genetic approaches to incorporate target analytes into the structure of the photoprotein. To that end, highly sensitive bioluminescence competitive binding assays for drugs, peptides, hormones, biomarkers, and vitamins have been developed. The assays developed employing Aquamax as a reporter/label are capable of quantitatively determining biomolecules at very low levels and in small volumes, even at the picoliter-volume scale and in single cells. For example, when Aquamax is used as a label in binding assays, it demonstrates excellent range. In addition, through the use of molecular biology SenseOmics prepares modified photoproteins with different wavelengths of emission for use in multianalyte detection and high throughput screening applications.
For package size, pricing and availability please contact us. We can customize your order to your requirements. REFERENCES: 1. J. C. Lewis and S. Daunert. "Photoproteins as Luminescent Labels in Binding Assays" Fresenius' Journal of Analytical Chemistry, 366(6-7), 760-768, 2000.
2. S. Ramanathan, J. Lewis, M. S. Kindy, and S. Daunert. "Heterogeneous Binding Assay for an Octapeptide Using Recombinant Aequorin." Analytica Chimica Acta, 369(3), 181-188, 1998.
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